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CFU-A

(colony-forming units) These cells are detected in an assay system using conditioned medium from rat AD-1-19T fibroblasts, which produces a cocktail of cytokines, mainly GM-CSF, M-CSF, SCF, small amounts of IL1, IL6, but no IL3.

Normal bone marrow cells give rise to large colonies (see also: Colony formation assay) in the presence of the cocktail of cytokines with high cloning efficiency.

The CFU-A assay detects multipotent hematopoietic stem cells that are also detected by the in vivo spleen Colony formation assay ... ... ... ... ... Subscribe to continue reading! ... ...

 

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