IER3 |
IETD-AFC |
RADHPF |
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The tetrapeptide sequence Ile-Glu-Thr-Asp corresponds to one of the caspase-8 cleavage sites (amino acids 172-175) of the inactive caspase-3 precursor. An enzyme that recognizes the IETD sequence is referred to sometimes also as IETDase and would thus be caspase-8 or an enzymer with caspase-8-like activity. Cleavage after the IETD site, together with cleavage at another site, yields the two subunits of bioactive caspase-3 during cell death by apoptosis (Nicholson et al, 1995).
IETD conjugated to 7-amino-4-trifluoromethyl coumarin (IETD-AFC) or 7-Amino-4-methyl coumarin (IETD-AMC) are fluorometric substrates for caspase-8
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