lining cells |
Lipasin |
TNF-alpha-inducing protein |
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This highly quiescent cell type has been isolated from murine bone marrow (Radley et al, 1999). They are very small in size and may represent a population of so-called small stem cells. The cells do not express lineage markers and have been isolated by virtue of their to exclude both Rhodamine-123 and Hoechst 33342 dyes. The analysis of the in vitro growth characteristics of these cells demonstrates an obligatory requirement for multiple growth factors, with 62 % of the sorted population having the capacity to form colonies in a colony formation assay in the presence of M-CSF + IL1-alpha + IL3 + SCF.
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