MS-5
The murine MS-5 stromal cell line has been established by Itoh et al (1989) after irradiation of the adherent cells in long-term BMC (bone marrow culture). MS-5 cells do not express alkaline phosphatase-, peroxidase-, and factor VIII, and are positive for periodic acid-Schiff and nonspecific esterase. The cells produce extracellular matrix proteins such as fibronectin, laminin, and collagen type 1.
The cells produce GM-CSF, and high quantities of IL6 and SCF (Suzuki et al, 1992). They also express HGF and the receptor, met
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