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TUNEL assay

[TUNEL-negative, TUNEL-positive]

[Terminal deoxynucleotidyl transferase mediated dUTP nick-end-labeling assay; TdT-dUTP terminal nick-end labeling assay] This assay has been designed to detect cells dying of apoptosis (see: cited references for overview). These cells are often being referred to as Tunel-positive cells.

This widely used and highly sensitive assay is based on the fact that nuclear DNA becomes fragmented by endogenous endonucleases during apoptosis and that characteristic, nonrandom DNA fragmentation is common to all cells undergoing this form of programmed cell death. Fragmented DNA ... ... ... ... ... Subscribe to continue reading! ... ...

 

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