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apoptosis assays

[apoptosis assay]

Different techniques have been developed to detect cell dying by the process of apoptosis and not from necrosis or other forms of cell death. The identification of the mode of cell death and its quantitation is not always simple or straightforward as the classification of apoptotic cells and apoptotic bodies as well as necrotic cells, miscalculation of the frequency of apoptosis and cell death rates, and a variety of inherent pitfalls of the experimental procedures used must be taken into account and may necessitate use of a combination of techniques (Darzynkiewicz et al, 1998). It should be noted that the many different morphologic variants of cell death ... ... ... ... ... Subscribe to continue reading! ... ...

 

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