Cytokines & Cells Online Pathfinder Encyclopaedia |
cytokine spot-forming cells |
B-cell adaptor containing src homology 2 domain |
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This assay is used to determine the frequencies of phenotypically identifiable antigen-reactive T-cells in order to obtain information about immunologic responses to tumor-associated antigens in phase 1 clinical trials of cancer vaccines. The assay relies on the tightly regulated production and secretion of cytokines by cells following cell activation by antigens, capture of a secreted cytokine (IFN-gamma), by an artificial cytokine-specific cell surface affinity matrix, and flow-cytometric detection of bound cytokine with a fluorochrome-labeled anti-cytokine monoclonal antibody (Mathioudakis et al, 2002).
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