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A32

The A32 is an antigen associated with melanoma cells and is defined by a murine monoclonal antibody. The antigen is an integral membrane glycoprotein of 113 kDa containing sialic acid and HNK-1 carbohydrate moieties. The antigen is present on most melanoma cells and melanoma nevi but not on normal epidermal melanocytes, and is seen also in endothelium, smooth muscle, cerebellum, and hair follicles. Tryptic peptides of the A32 antigen show sequence identity with the MUC18 antigen. Melanoma cells adhere to affinity-purified A32 antigen. Adhesion is blocked by either soluble A32 antigen or monoclonal antibody against the HNK-1 carbohydrate moiety (Shih et al, 1994).

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