FEDLPNFGHIQVKVFNHGEHIHH |
feeding circuit-activating peptide a |
very early activation |
||
[feeder cell]
Feeder cell layers usually consist of adherent growth-arrested but viable and bioactive cells (primary cells or continuous cell lines) that have been incapacitated, for example by irradiation. These cells are used as a substratum on which other cells are grown in a co-culture system. Sometimes, the term filler cells is used.
Some cells can be grown in culture dishes, coated, for example, with collagen, fibronectin, or a mixture of the extracellular matrix components (Matrigel). Many cell types, however, are completely dependent on physical contact with a feeder layer for survival and expansion. Feeder cell layers provide an intact and functional
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