Gene family |
gene knock-out |
M1 cells |
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[library, gene libraries, cloned, genomic clone, genomic clones, cloning]
A term used to describe a collection of DNA fragments derived from the genome of an organism and cloned randomly into suitable cloning vectors (plasmids, phages). If plasmid cloning vectors are used for the establishment of the library, this library essentially is a collection of host cells, each of which contains a plasmid with an inserted DNA fragment. If phages have been used to clone the DNA fragments, the library consists of a phage lysate with each phage containing an inserted fragment of DNA. Together the individual fragments in the collection of inserted molecules represent the entire genetic information contained in an organism and in this case the library is said to be a representative library.
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