32D

An immortalized myeloblast-like cell line originally derived from long-term cultures of murine bone marrow (see also: BMC) supplemented with WEHI-3B conditioned medium. The most frequently employed subline is 32D clone 3 (32D cl3).

32D cells are characterized by a lack of extracellular matrix components and they can be propagated in suspension (see also: Cell culture). Cells can be kept indefinitely as undifferentiated cells as long as they are stimulated by IL3. Removal of IL3 arrests them in the G0/G1 phase of the cell cycle and cell growth is synchronized upon renewed addition of ... ... ... ...
 
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Entry last modified: March 2002