Ala(8,13,18)-magainin-2 amide |
Ala-Met-Pro-Lys-Pro-Trp |
VKLIESKEAFQEALAAAGDKL |
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AlamarBlue is a redeox indicator that yields a colorimetric change and a fluorescent signal in a response to a metabolic activity. The compound is nontoxic, soluble and stable in tissue culture medium, and has been used as an alternative to the MTT assay and proliferation assays based on the incorporation of tritiated thymidine, allowing the quantitative measurement of the proliferation of a variety of human and animal cells, bacteria, mycobacteria, and fungi under various conditions (Ahmed et al, 1994; Back et al, 1999; de Fries et al, 1995; Gloeckner et al, 2001; Gray et al, 1996; Nakayama et al, 1997; Voytik-Harbin et al, 1998). The assay can be used for cell suspensions or attached cells (see also: cell culture). It has been used also to measure the proliferation of cells in response to various
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